หน้าแรก ตรวจหวย โปรโมชั่น เว็บบอร์ด ควิซ Pic Post
 
Page หาเพื่อน Chat หาเพื่อน Line หาเพื่อน Skype
 
อัลบั้ม แต่งรูป คำคม Glitter สเปซ ไดอารี่
 
เกมถอดรหัสภาพ เกม วิดีโอ
 
คำนวณ
 
ติดต่อเว็บไซต์ลงโฆษณาลงข่าวประชาสัมพันธ์แจ้งเนื้อหาไม่เหมาะสมเงื่อนไขการให้บริการ
 
Login เข้าสู่ระบบ สมัครสมาชิก
 
New Method for Profiling of DNA Methylation and Chromatin Structure in single cells
16:15 - 24 มีนาคม 2563

The DNA methylation status in cells is significantly different in different types of cells, and its dynamic regulation may be closely related to the structure of chromatin in the nucleus. There are already available technologies for sequencing DNA methylation (whole genome bisulfite sequencing, WGBS) and chromatin structure (Hi-C) in the genomics field. However, due to fragment length limitations, traditional whole-genome bisulfite sequencing cannot effectively detect multi-site coordinated DNA methylation.

A few months ago, researchers from the U.S. University of California published an article titled Joint profiling of DNA methylation and chromatinarchitecture in single cells in Nature Methods, reporting a newly developed technology called Methyl-HiC enables simultaneous DNA methylation and chromatin structure determination.

Methyl-HiC's technology uses common Hi-C procedures to obtain DNA fragments, and then adds a bisulfite conversion step before library construction, and then performs sequencing. The researchers first practiced the technique on cultured mouse stem cells. They found that the contact matrix produced by Methyl-HiC was highly similar to that of Hi-C. The chromatin loops and topological domains (TADs) produced by the two are basically the same. Compared with WGBS, Methyl-HiC deleted about 20% of CpG due to the selective acquisition of interacting DNA fragments by Hi-C, and was more enriched in the open chromatin region. These results show that it is basically feasible to combine Hi-C and WGBS.

The researchers then examined the relationship between DNA methylation and chromatin structure. They found that there was a correlation between the status of CpG methylation at both ends of the chromatin loop. If CpG is in the same TAD or open chromosomal region, the correlation will be higher. At the same time, the degree of DNA methylation is also related to its chromatin status.

The unique advantages of Methyl-HiC technology are even more apparent in single-cell research. Due to cellular heterogeneity, it is often necessary to measure various epigenomics of a single cell. However, traditional technology cannot obtain WGBS and Hi-C data of the same cell at the same time. Using scMethyl-HiC (singlecell Methyl-HiC), the authors measured DNA methylation groups and three-dimensional genomes of multiple mouse stem cells cultured in serum and 2 medium, respectively. After addition, scMethyl-HiC is similar to the contact matrix produced by previous single-cell Hi-C. The number of CpG is also basically consistent with the result of single cell WGBS. The researchers then used data from the methylation group in scMethyl-HiC to cluster stem cells under these two culture conditions, and found that stem cells cultured in serum media could be divided into two additional categories. Observation of the contact matrix of these three types of cells also revealed significant differences. Many of these genes with different methylation levels and chromatin interactions are related to the development of limbs, such as Epha4 and HoxD.

The author also mentioned that the current data generated by scMethyl-HiC is sparse. However, the current results show that scMethyl-HiC technology is valuable in studying cell-specific chromatin structure in complex tissues.

คอมเม้นต์
กรุณา "เข้าสู่ระบบ" ก่อนคอมเม้นต์
ผู้เขียน
บล็อกโปรด